论文题目：Hydrogen peroxide biosensor based on microperoxidase-11 immobilized on flexible MWCNTs-BC nanocomposite film

所属学科：功能高分子

论文作者：Bingyan Zhang, Jianhai Zhou, Shaohui Li, Xiaofan Zhang, Mingkui Wang, Guang Yang*, Yan Shen*

关键字：Biosensor，Bacterial cellulose，Multi-walled carbon nanotubes; Microperoxidase-11，H2O2 detection

论文来源：期刊

具体来源：Talanta, 2015, 131: 243-248.

发表时间：2015.4

摘要：

In the present work, we report on an experimental study of flexible nanocomposite film for electrochemical detection of hydrogen peroxide (H₂O₂) based on bacterial cellulose (BC) and multi-walled carbon nanotubes (MWCNTs) in combination with microperoxidase-11 (MP-11). MWCNTs are used to functionalize BC and provide a flexible conductive film. On the other hand, BC can improve MWCNTs? biocompatibility. The investigation shows that MP-11 immobilized on the flexible film of MWCNTs–BC can easily present a pair of well-defined and quasi-reversible redox peaks, revealing a direct electrochemistry of MP-11 on the nanocomposite film. The apparent heterogeneous electron-transfer rate constant k_s is estimated to be 11.5 s^?1. The resulting flexible electrode presents appreciated catalytic properties for electrochemical detection of H₂O₂, comparing to traditional electrodes (such as gold, glassy carbon electrode) modified with MP-11. The proposed biosensor exhibits a low detection limit of 0.1 μM (at a signal-to-noise ratio of 3) with a linear range of 0.1–257.6 μM, and acquires a satisfactory stability.