设为首页  |  加入收藏  | 联系我们
重要通知:
科研成果Enrollment
Hot News / 热点新闻
2024 - 01 - 26
点击次数:
华中科技大学刘长清团队、杨光教授团队联合博士后招聘启事一、团队负责人简介刘长清,华中科技大学机械...
2023 - 11 - 24
点击次数:
2023年10月13日至17日,由江明院士倡议并策划,杨光教授、陈红教授组织协调的“高分子学人书画邀请展”...
2023 - 08 - 20
点击次数:
由中国化学会主办,中国化学会纤维素专业委员会、贵州大学共同承办的“中国化学会第3届全国纤维素学术研...
2023 - 08 - 11
点击次数:
2023年8月9-11日,中国化学会第三届全国纤维素学术研讨会在贵阳市顺利召开,此次学术研讨会主题为“创新...
2023 - 08 - 01
点击次数:
织物型纳米摩擦发电机(Fabric-based TENGs)具有开发为织物型可穿戴器件的前景,其出色的输出性能、柔...
2023 - 04 - 19
点击次数:
2023年4月7日-4月9日,华东交通大学材料学院等单位主办的第一届生物材料与医疗器械赣江学术论坛在江西南...
2023 - 04 - 19
点击次数:
2023年4月11日晚上八点到十点半,华中科技大学与多伦多大学联合举办了“面向人类福祉的创新与未来技术”...
2023 - 03 - 28
点击次数:
2023 年 3 月 18 日, MDPI 作者培训会在华中科技大学生命科学与技术学院东十一楼 221 会议室召开。IJMS...
微博 / WeiBo
Enrollment 科研成果
Encapsulation of E. coli in biomimetic and Fe3O4 doped hydrogel: Structural and viability analyses
日期: 2017-11-26
浏览次数:
标题:Encapsulation of E. coli in biomimetic and Fe3O4 doped hydrogel: Structural and viability analyses

摘要:     The current study reports the modification of prokaryotic microorganism through a single layer technique by using different polyanions/cations and doping with magnetic (Fe3O4) nanoparticles. Briefly, individual Escherichia coli cells were encapsulated through deposition of 1% sodium alginate as first layer followed by depositing precipitate layers of calcium chloride, disodium hydrogen phosphate, and Fe3O4 nanoparticles. Surface and cross sectional analysis of modified E. coli cells by field emission scanning electron microscope (FE-SEM) confirmed the synthesis of varying sizes of artificial shells around the microbial cells while the deposition of Fe3O4 nanoparticles was confirmed by transmission electron microscope (TEM). Thermogravimetric analysis (TGA) showed the deposition of 58 wt% of Fe3O4 nanoparticles on E. coli cell surface. Chemical structure analysis by fourier transform infrared (FTIR) spectroscopy confirmed the presence of characteristic functional groups of deposited reagents in the hydrogel capsule. Zeta potential analysis of hydrogel capsule showed moderate stability with a surface charge of -21 mV. Growth and viability analysis by Alamar Blue assay indicated marked increase in the reduction of resazurin blue (>100%) by the modified E. coli indicating their viability. The movement and control of magnetized E. coli cells was manipulated using external permanent magnetic field as observed with optical microscope images. The surface modified cells can find potential applications in bioremediation, biodegradation, catalysis, and can be used as biosorbents.


Encapsulation of E. coli in biomimetic and Fe3O4 doped hydrogel: Structural and viability analyses. Available from: https://www.researchgate.net/publication/321105532_Encapsulation_of_E_coli_in_biomimetic_and_Fe3O4_doped_hydrogel_Structural_and_viability_analyses [accessed Nov 26 2017].

 期刊:Appl Microbiol Biotechnol

Online avaible:2017.11.03


地址:武汉市洪山区珞瑜东路1037号华中科技大学东11楼生命科学与技术学院

版权所有  华中科技大学. 保留所有权利