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Enrollment 科研团队
SALHI MARIEM
日期: 2020-03-25
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基本信息

Name:

SALHI MARIEM

enrollment year

2018

Boy/Girl:

Girl

Master/Ph.D

Ph.D

Hometown:

Tunisia

Birth Date:

21/01/1990

Hobby:

Dance and reading book

Education   Background

Time

Bachelor/Master/Ph.D

School

Major

2008-2011

Bachelor

Faculty of Science Tunisia

Life Science

2011-2013

Master

Faculty of Science Tunisia

Molecular Biology and   Biotechnology

Working Experience

Time

Position

Address

Remarks

2014

Medical Lab technician

Tunisia

Biochemistry

2015-2018

Lab Assistant

Faculty of Medicine   Tunisia

Genetics

Researching   Direction

11-12/2017           Internship at ICBAS University of Porto, Portugal

Basic techniques   for culturing mammalian cells

Preparation of   cell culture media;

Cryopreservation   (freezing and thawing of cell lines);

Trypan Blue test   for cell viability;

SRB test for   cell proliferation;

MTT Assay for   cellular cytotoxicity

09/2016-02/2017   Visiting student at Huazhong University of   Sciences and Technology. Wuhan; China

Study development in Zebrafish Model

In situ Hybridization/ O-staining

Microinjection of Morpholino

RNA extraction and Reverse Transcription

Plasmid construction

11/2015 Internship at Srebrnjak Children Hospital Zagreb,   Croatia.

Introduction to Flow Cytometry technique

Genetic study of gene GLCCI1 by qPCR

06/2015   Training Session on Laboratory Animals

National School of Veterinary Medicine,   June 2015:

Conferences: Ethics and Wellness AL / Principle   of 3Rs / Anesthesia and Euthanasia

Workshops: Practical demonstrations on   models: Rabbit and Guinea Pig / Rat and Mouse.

02/2013- 12/2014 Master   Project: Genetic Polymorphisms of Toll like Receptors (TLR2, TLR4) and CD14   and susceptibility to asthma in the Tunisian population.

o Removal blood

o Extraction of genomic DNA by the method   "Salting Out"

  Preparation of lysis solutions  

o PCR and q PCR

Researching   Achievement

Papers or patents:

Mariem   Salhi, Oussama Lahmar, Marwa Ouled Salah, Ivana Banic, Bao Binghao, Waqar   Malik, Kamel Hamzaoui, Mirjana Turkalj and Agnes Hamzaoui. GLCCI1 and STIP1 variants are associated with asthma   susceptibility and inhaled corticosteroid response in a Tunisian population. Journal of Asthma. 2019;1-10. Doi: 10.1080/02770903.2019.1666867.

Meetings attended

Cancer and cell aging wuhan

Allergology meeting Tunisia

honor

certificate

Contact information

Adress

湖北省武汉市华中科技大学生命科学与技术学院J2

Email:

Mariam.salhi2012@gmail.com

Tel

+216 92992357// +86   13163296856








 

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